Screen Printing Bio-chip Sensor for Cholesterol Detection Based on Molecular Imprinting Self-assembled Film
Xue Qian-nan①② Bian Chao① Ren Zhen-xing①② Sun Ji-zhou① Bian He-ming①② Han Jing-hong① Xia Shan-hong①
①(State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China) ②(Graduate University of Chinese Academic of Sciences, Beijing 100039, China)
Abstract：An electrochemical bionic sensor for cholesterol detection is developed with molecular imprinting self-assembled film deposited on the screen printed gold electrode. The surface topography of planar bare gold electrode and thick film bare gold electrode are compared with Scanning Electron Microscope (SEM). The electrochemical characteristics of the electrode during modification are studied with cyclic voltammetry technique. The results show that thick film electrode by screen printing technology is suited to the modification of molecular imprinting self-assembled film, and exhibits obvious amplification at nano level. The response of the sensor to the concentration of cholesterol is detected with chronoamperometric measurements. Cholesterol between 0 and 700 nM are detected with this sensor. The linearity range is from 50 nM to 700 nM with the sensitivity of -4.94 μA/[lg(nM)] and linearly dependent coefficient of 0.994. And this cholesterol sensor has high accuracy, which reaches 99.56%.
2010, Vol. 32 
